Extended Data Fig. 2: Effect of Calyculin A and H1152 on Myosin II phosphorylation and apical cell area.
From: Self-organized tissue mechanics underlie embryonic regulation
(a-c) Control (a), Calyculin A- (b) and H1152-treated (c) embryos immunostained using anti-pMyosin II antibodies (cyan). (d-l) Higher magnification in the posterior margin region showing pMyosin II (cyan) and ZO-1 (magenta) staining in control (d, g, j), Calyculin A- (e, h, k), and H1152- (f, i, l) treated embryos. (m-o) Colour-coded apical area of cells segmented using the ZO-1 staining. (p) Quantification of cell area; Calyculin A: n = 4 biologically independent embryos, 1914 cells; Control: n = 4 biologically independent embryos, 1059 cells; H1152: n = 3 biologically independent embryos, 629 cells; Mean ± s.e.m; *p = 0,0193, ****p < 0.0001; Nested one-way ANOVA.
