Extended Data Fig. 5: Differences in projection patterns of P1 subpopulations.
From: A modular circuit coordinates the diversification of courtship strategies
a, Diagram of the subdivision of the pC1 neurons by Dsx- (purple) and Fru-expression (red). Also noted is the subset of pC1 neurons captured by the P1-specific driver used in this study and the populations labeled through genetic intersection of this P1 driver with Dsx or Fru. Note that the labeled populations are not to scale, but precise neuron counts labeled in each intersection are provided in b-e. b-e, (Left) Unmasked images of Fru∩P1 and Dsx∩P1 neurons labeled by intersection of Fru (b,d) or Dsx (c,e) and the P1-driver 71G01 (Fru∩P1 and Dsx∩P1, respectively) in D. melanogaster and D. yakuba. Stained for GFP (green) and neuropil counterstain (magenta). (Right) Representative images of anti-Dsx (purple) and anti-Fru (red) staining used to count Dsx+ and Fru+ subsets and quantification of Dsx+ Fru∩P1 neurons and Fru+ Dsx∩P1 neurons within the Dsx+ pC1 cluster, based on anti-Dsx and anti-Fru immunostaining, respectively. Data points represent cell number in one hemisphere and both hemisphere counts are plotted separately (Sample sizes: mel Fru∩P1 n = 7; mel Dsx∩P1 n = 8; yak Fru∩P1 n = 7; yak Dsx∩P1 n = 5). f,g, Comparison of the projections of Fru∩P1 and Dsx∩P1 subpopulations across subtype (f) or across species (g). Heatmap (left) and skeletonized overlays (right) of the comparison groups. Regions of greatest difference across both subtype and species indicated (cyan triangle) and region of significant difference between Dsx∩P1 and Fru∩P1 in D. yakuba noted (yellow arrow). All images are dorsal side up.
