Extended Data Fig. 6: Subspecialization of P1 types in D. melanogaster.
From: A modular circuit coordinates the diversification of courtship strategies
a,b, (Left) Maximum intensity projections of neurons labeled by intersection of Fru (a) or Dsx (b) and the P1-driver 71G01 (Fru∩P1 and Dsx∩P1, respectively) in D. melanogaster, registered to a common template brain (see methods). (Middle) Averaged functional responses (∆F/F0) aligned to time of a tap of indicated conspecific or heterospecific female and average peak response (∆F/F0) per male for a given female target (n = 10 each). (Right) Average courtship bout length, total percent time pursuing, or total percent time extending a unilateral wing toward a D. suzukii female target prior to (Pre) or during (Stim) optogenetic stimulation of Fru∩P1>CsChrimson or Dsx∩P1>CsChrimson males and the fraction of flies engaging in these behaviors over the course of the experiment. (n = 10 each) c, Functional responses of the or Dsx+ neurons innervating the LPC where P1 neurons reside with or without fru-Gal80. (Left) Diagram of the P1/pC1 neural cluster illustrating the nested nature of the Fru+ contingent within the broader Dsx+ population. Green highlighting represents which subsets express GCaMP in the different genetic backgrounds (all Dsx+Fru- and Dsx+Fru+ pC1 neurons in dsx>GCaMP flies, but only the Dsx+Fru- subset in the dsx>GCaMP; fru-Gal80 flies). Also, representative images Dsx+ neural processes and somata expressing GCaMP (green) and counter-stained for Fru (red). Note the denser projections and the presence of Fru+ GCaMP+ somata in the dsx>GCaMP animals, which is absent in animals expressing fru-Gal80. (Right) Averaged functional responses (∆F/F0) aligned to time of a tap of indicated conspecific or heterospecific female and (Right) average peak response (∆F/F0) per male for a given female target. (Sample sizes: dsx>GCaMP n = 6; dsx>GCaMP;fru-Gal80 n = 7). For functional imaging (a-c), shading represents mean ± SEM. Points are individual males, error bars are mean ± SEM. For behavioral tests (a,b), points are individual males. Statistics: ANOVA with Tukey’s post-hoc (a-c) or Wilcoxon test (a,b). Letters denote statistically different groups (p < 0.05). Asterisks: *P < 0.05, **P < 0.01, ***P < 0.001.
