Extended Data Fig. 8: Multimodal sensory integration by P1 facilitates sex discrimination in D. yakuba.
From: A modular circuit coordinates the diversification of courtship strategies
a, Percent time a D. melanogaster or D. yakuba male spends courting one target over the other when presented a choice between a conspecific female and male or a conspecific female and a heterospecific female that shares the same pheromone profile (D. simulans) (n = 10 each). b, CRISPR/Cas9 targeting strategy for the genetic deletion of the OR67d locus and validation by PCR. c, Average courtship bout lengths of a wildtype or OR67d mutant D. yakuba male directed toward another D. yakuba male (n = 20 each). d, Average courtship bout lengths of a D. yakuba male toward a D. yakuba female mock perfumed or perfumed with 7-T, 7,11-HD, or cVA (n = 10 each). e, (Left) Averaged functional responses (∆F/F0) aligned to time of a tap of D. yakuba females perfumed as in c and average peak response (∆F/F0) per male for a given female target (n = 7). f,g, Average courtship bout lengths of a wildtype or deafened (arista removed) D. yakuba male directed toward another D. yakuba male (f; n = 20 each) or a wildtype D. yakuba male toward a wildtype or muted (wings removed) D. yakuba male (g; intact n = 20, muted n = 18). h, Diagram summarizing the multisensory cues the D. yakuba males rely upon to mediate conspecific attraction and inhibit heterospecific and intrasexual courtship. For behavioral tests (a,c,d,f,g), points represent individual males, bars are median. For imaging (e), points are individual males, error bars are mean ± SEM. Statistics: Wilcoxon test (a,d), unpaired Mann-Whitney (c,f,g), or ANOVA with Tukey’s post-hoc (e). Letters denote statistically different groups (p < 0.05). Asterisks: *P < 0.05, ns, not significant.
