Extended Data Fig. 3: Conserved anatomy of pheromone-detecting sensory neurons.
From: A modular circuit coordinates the diversification of courtship strategies
a, CRISPR/Cas9 targeting strategy for the genetic deletion of the ppk23 locus and validation by PCR and ie1-mCherry marker expression. b,c,d, Percent time courting in the 10 min following courtship initiation, courtship latency, and courtship bout length of ppk23 mutants relative to wildtype for D. melanogaster in the dark (b) and D. yakuba males in the light (c) or in the dark (d) (n = 20 each). Average Bout Lengths are re-plotted from Fig. 3 for reference. e, Representative bright field images and quantification showing expression of CD8::GFP (green) in the soma of the Ppk23+, Fru+, and Dsx+ sensory neurons in the foreleg tarsal segments of D. melanogaster and D. yakuba males. Images are distal end up. f, (Left) Average functional responses (∆F/F0) aligned to time of a tap (as in Fig. 3) of indicated D. melanogaster (oe+ ctrl), mock-perfumed oenocyte-less (oe-), 7,11-heptacosadiene-perfumed (oe- +7,11-HD), 7-tricosene-perfumed (oe- +7-T), or cis-Vaccenyl acetate-perfumed (oe- +cVA) female of the foreleg sensory afferents and (Right) average peak response (∆F/F0) per male for a given female target (n = 5). g, Functional responses of Dsx+ neurons in the LPC (as in Fig. 4d) in ppk23 mutant males (n = 6)). For behavioral tests (b-c), points represent individual males, bars are median. For anatomy (e), points represent individual forelegs, bars are mean ± SEM. For imaging (f,g), shading is mean ± SEM, points are individual males, error bars are mean ± SEM. Statistics: ANOVA with Tukey’s post-hoc (f,g) or unpaired Mann-Whitney (b-d). Letters denote statistically different groups (p < 0.05). Asterisks: **P < 0.01, ****P < 0.0001, ns, not significant.
