Extended Data Fig. 4: Ppk25+ Sensory neurons promote courtship in the dark in D. yakuba.
From: A modular circuit coordinates the diversification of courtship strategies
a,b, CRISPR/Cas9 targeting strategy for the genetic deletion of the ppk25 locus and validation by PCR (a) and courtship in the dark (as in Extended Data Fig. 3) toward D. yakuba females (b). Average courtship bout lengths are re-plotted from Fig. 3 for reference (n = 20). c, Replotting of sensory responses in D. yakuba wildtype and ppk23 and ppk25 mutant males from Fig. 3e for statistical comparison. For behavioral tests (b), points represent individual males, bars are median. For imaging (c), points are individual males, error bars are mean ± SEM. Statistics: Unpaired Mann-Whitney (b) or ANOVA with Tukey’s post-hoc (c). Letters denote statistically different groups (p < 0.05). Asterisks: ****P < 0.0001, ns, not significant.
