Extended Data Fig. 5: CXCL17 is a chemoattractant ligand for GPR25 but not GPR15 or CMKLR1.
From: A lymphocyte chemoaffinity axis for lung, non-intestinal mucosae and CNS
a. Human GPR15 transfectants migration to GPR15LG (250 nM) and CXCL17 (10–300 nM). b. Human CMKLR1 transfectants migration to chemerin and CXCL17 (10–300 nM). c. Checkerboard assay with human 4CysCXCL17 250 nM and human GPR25 transfectants. d. Pertussis toxin (100 ng/ml, 2 h pre-treatment before migration assay) inhibits CXCL17-induced chemotaxis on human GPR25 L1-2 transfectants. e. Intact mouse 6CysCXCL17 (3 nM - 1μM) is an active chemoattractant on human GPR25. f. Intact human 4CysCXCL17 is an active chemoattractant on mouse GPR25. g. mGPR25 transduced cells, but not the empty vector transduced counterparts robustly chemotax to mouse and human CXCL17 in in vitro transwell-based migration assays. Results with 3–9 replicates pooled from at least two independent experiments are shown as mean ± SEM. ****; P < 0.0001 vs no chemokine control in a two-tailed T-test.
