Extended Data Fig. 5: Mutations in independent nucleotide binding sites of SBS Tad1 disrupt specific inhibitory activities.
From: Single phage proteins sequester signals from TIR and cGAS-like enzymes
a, Plaque assay to test the activity of SBS Tad1 against Thoeris immunity in vivo. F10 phage was spotted in 10-fold serial dilutions on a lawn of P. aeruginosa cells expressing Thoeris operon genes (PAO1:Tn7 Thoeris SIR2), or without Thoeris (PAO1:Tn7 empty), electroporated with pHERD30T plasmids carrying SBS tad1 (wild type or mutant gene) or empty vector. b, Plaque assay to test the activity of SBS Tad1 against CBASS II-AGA immunity in vivo. PaMx41∆acb2 was spotted on a lawn of Pa011 cells with deletion of CBASS operon (Pa011ΔCBASS II-AGA) or Pa011 wild type cells (Pa011 wt), electroporated with pHERD30T plasmids carrying SBS Tad1 (wild type or mutant gene) or empty vector. c, Plaque assay to test the activity of SBS Tad1 against CBASS III-CAAA immunity in vivo. JBD67Δacb2 phage was spotted in 10-fold serial dilutions on a lawn of P. aeruginosa cells expressing Pa278 CBASS operon genes (PAO1:Tn7CBASS III-CAAA), or without the system (PAO1:Tn7 empty), electroporated with pHERD30T plasmids carrying SBS tad1 (wild type or mutant gene) or empty vector.
