Extended Data Fig. 7: SPO1 Tad2 does not bind to cyclic dinucleotides.
From: Single phage proteins sequester signals from TIR and cGAS-like enzymes
a, Native PAGE showed the binding of SPO1 Tad2 to cyclic nucleotides and gcADPR molecules. For gel source date, see Supplementary Fig. 1. b, The ability of SPO1 Tad2 to bind and release 3′,3′-cGAMP when treated with proteinase K was analysed by HPLC. 3′,3′-cGAMP was used as a control. The remaining nucleotides after incubation with SPO1 Tad2 was tested. c, Overlay of sensorgrams from surface plasmon resonance (SPR) experiments, used to determine kinetics of SPO1 Tad2 binding to cyclic dinucleotides. Data were fit with a model describing one-site binding for the ligands (black lines).
