Extended Data Fig. 8: HgmTad2 binds to cGG and gcADPR molecules.
From: Single phage proteins sequester signals from TIR and cGAS-like enzymes
a, Profile of ion exchange chromatography of HgmTad2 using Resource Q column (1 mL, GE Healthcare). Proteins in peaks 1–3 are collected separately and marked as State 1–3. The proteins in three states were then subjected to native PAGE and SDS-PAGE, respectively. b, Native PAGE showed the binding of HgmTad2 in three states to 1″,2′ gcADPR. For gel source date of a and b, see Supplementary Fig. 1. c, SPR analysis of HgmTad2 binding to 2′,3′-cGAMP. The data was fitted with affinity model and the calculated KD was shown.
