Extended Data Fig. 8: Single-cell RNA-seq profiles of AOM/DSS-induced colon neoplasms.
From: Polyclonal-to-monoclonal transition in colorectal precancerous evolution
a, Diagram of uniform manifold approximation and projection (UMAP) showing the integration of single-cell RNA-seq data from wild-type (WT) normal samples33 (n = 2) and AOM/DSS neoplasms in this study (n = 9). b, UMAP plot showing the single cells colored by individual samples. c, UMAP plot showing the single cells colored by annotated cell types. d, Proportion of cell types in each sample ordered by increase of lesion clonality (1/Np), where Np represents the number of founding progenitors. e, Spearman’s correlation between the proportion of macrophages, neutrophils, endothelial or epithelial cells and the lesion clonality (1/Np). The shaded region represents 95% CI of regression. Spearman’s ρ and P value are shown. d, Proportion of cell subtypes in each sample ordered by increase of lesion clonality (1/Np). g, Hallmark pathway analysis of differentially expressed genes between high clonality versus low clonality AOM/DSS neoplasms. Late and early lesions are defined as Np ≤ 3 and >3, respectively. NES, normalized enrichment score.
