Extended Data Fig. 1: Establishment of AOM/DSS and ApcMin/+ mouse models carrying the SMALT lineage tracing system.
From: Polyclonal-to-monoclonal transition in colorectal precancerous evolution
a, The 3 kb DNA barcode consists of 16 different tandem targets, each with an 18 bp iSceI binding site and a 156 bp editing region. The HsAID protein mainly induces C-to-T (or G-to-A) mutations in the editing region, which can be used to reconstruct single-cell phylogenies. b, The SMALT system was knocked into the H11 locus of C57BL/6 J mouse. The insert contains hsAID, iSceI and a 3 kb DNA barcode. c, Schematic of our mouse experiments following standard dose and course of drug treatment. d, Mouse absolute weight (top) and relative weight to initial timepoint (bottom) were recorded over time in days. Error bars presented as mean ± SEM. e, At the endpoint of experiment, normal colons, AOM/DSS colons or whole small intestines in ApcMin/+ mice were dissected.
