Fig. 3: Adipocyte promoters retain an epigenetic memory.
From: Adipose tissue retains an epigenetic memory of obesity after weight loss
a, Experimental setup of the WL study in AdipoERCre x NuTRAP mice. b, Workflow of paired CUT&Tag, ATAC–seq and TRAP–seq from one AT depot. Biotinylated nuclei and GFP-tagged ribosomes are isolated from frozen tissue, pulled down and subjected to CUT&Tag, ATAC–seq (nuclei) and TRAP–seq (ribosomes). c, PCA of translatome (TRAP–seq) of labelled adipocytes from C, CC_s, CC_l, CCC, H, HH, HC and HHC. Each dot represents an individual biological replicate. d, MOFA plots showing the sample clustering along latent Factors 1 and 2 (left) and Factor 1 value distribution (right) across labelled adipocytes. Each dot corresponds to one biological replicate. For each replicate all six modalities are represented in one dot. e, Percentage of variance explained by each MOFA factor across one of six modalities. f, Dynamics of differentially H3K4me3-marked promoters (y axis) from H to HC. g, Dynamics of differentially H3K27me3-marked promoters (y axis) from H to HC. h, Scaled enrichment of H3K4me3 (left), H3K27me3 (middle) and H3K27ac (right) at selected promoters of genes and the log2FC of TRAP–seq from comparisons against controls for the same genes. i, Distribution of normalized reads of H3K4me3 and H3K27me3 at the Cyp2e1 and Icam1 loci across conditions. Scaling of reads was performed per hPTM. NS, not significant; v, versus.
