Extended Data Fig. 1: Human AT retains cellular transcriptional changes after bariatric surgery induced WL.
From: Adipose tissue retains an epigenetic memory of obesity after weight loss
a, UMAP of 19,494 nuclei representing omAT pools from lean subjects (n = 5; 1 male, 4 females) and paired omAT from T0 and T1 (n = 8 each; 2 males, 6 females) from the MTSS study. b,c Proportion of retained transcriptional changes in highly abundant cell types of MTSS omAT. d, UMAP of 31,721 nuclei representing scAT pools from lean subjects (n = 8; 8 females) and paired scAT from T0 and T1 (n = 7 each; 7 females) from the NEFA study. e,f Proportion of retained transcriptional changes in highly abundant cell types of NEFA scAT. g-j Number of upregulated and downregulated DEGs per cell type obese donor scaled by column at T0 for omAT (left) and scAT (right) from MTSS, LTSS and NEFA studies. k, Number of persistently deregulated genes from T0 to T1 per cell type across AT pools from all studies. l, UMAP of 4,958 nuclei representing adipocytes from MTSS omAT and LTSS omAT (total lean n = 10; total T0/T1 n = 13). m, UMAP of 13,231 nuclei representing adipocytes from NEFA scAT and LTSS scAT (total lean n = 13; total T0/T1 n = 12). Wilcoxon Rank Sum test, with adjusted p-value < 0.01 by the Bonferroni correction method and FC > ±0.5 was used for DEG identification in b, c, e-k. APCs, adipocyte progenitor cells; ASDCs, AXL+ dendritic cells; DCs, dendritic cells; EndoCs, endothelial cells; EndoACs, arteriolar EndoCs; EndoSCs, stalk EndoCs; EndoVCs, venular EndoCs; LECs, lymphatic endothelial cells; FAPs, fibro-adipogenic progenitors; Macro, macrophages; MastCs, mast cells; MesoCs, mesothelial cells; NeurCs, neuronal like cells; SMCs, (vascular) smooth muscle cells.
