Extended Data Fig. 2: Effects of initial mixing ratio and [(NH₄)₂SO₄] on concentric partitioning.

a, Optical microscopic images of a concentrically partitioned 23:77 mixture of ^MePEG_5K^NBD and ^MePEG_5K^NH2: (i) Confocal laser scanning microscopy (CLSM) image for λ_ext = 488 nm (NBD), (ii) bright-field image, (iii) phase contrast microscopy image, (iv) differential interference contrast microscopy image, and (v) three-dimensionally reconstructed CLSM image. Scale bars, 10 µm. Note: The mixing ratio was confirmed by ¹H NMR, where the unreacted residue ^MePEG_5K^NH2 for the synthesis of ^MePEG_5K^NBD was included as shown in b. b, CLSM images for λ_ext = 488 nm (NBD) (upper) of ^MePEG_5K^NH2 and ^MePEG_5K^NBD with different mixing ratios under the conditions for the concentric partitioning, together with their magnified CLSM images (middle) and normalized fluorescence intensity distributions along the pink-colored broken lines (lower). Ratios (weight): (i) 90/10, (ii) 75/25, (iii) 50/50, (iv) 25/75, and (v) 0/100. White scale bars, 10 µm. Right inset: three-dimensionally reconstructed CLSM image of the sample in (v). Yellow scale bar, 2 µm. c, CLSM images for λ_ext = 488 nm (NBD) of a 1:1 mixture of ^MePEG_5K^NBD and ^MePEG_5K^NH2 under the conditions for concentric partitioning using different concentrations of (NH₄)₂SO₄ (upper bar), together with their normalized fluorescence intensity distributions along the pink broken lines. Scale bars, 10 µm.