Extended Data Fig. 6: Anatomy of TuTuA cell types.
From: Social state alters vision using three circuit mechanisms in Drosophila
(a–e) Neuronal skeletons of the termini of the contralateral axons of TuTuAs from the hemibrain v1.2.1 connectome. (a) TuTuA_1 and TuTuA_2 are shown together (body IDs 676836779 and 5813013691, respectively). (b) TuTuA_1 (body ID 676836779) shown alone. (c) Same as panel a, but rotated 90 degrees along the medial-lateral axis. (d) TuTuA_2 (body ID 5813013691) shown alone. (e) Same as panel c, repeated to facilitate comparison. These anatomical differences were used to determine the correspondence between GAL4 driver lines and TuTuA subtypes. (f) Terminus of a contralateral axon of a neuron from GAL4 driver line SS77402 obtained by stochastic labelling70 and then segmented using VVD viewer (see Supplementary Table 1). (g) The comparison between the GAL4 driver line in f to TuTuA_1 skeleton shown in b. (h) Same as panel g, but rotated 90 degrees along the medial-lateral axis. (i) The comparison between the GAL4 driver line in f to TuTuA_2 skeleton shown in d. (j) Same as panel i but rotated 90 degrees along the medial-lateral axis. (k) Terminus of a contralateral axon of a neuron from GAL4 driver line SS77462 obtained by stochastic labelling70 and then segmented using VVD viewer. (l) The comparison between the GAL4 driver line in k to TuTuA_1 skeleton shown in b. (m) Same as panel g, but rotated 90 degrees along the medial-lateral axis. (n) The comparison between the GAL4 driver line in k to TuTuA_2 skeleton shown in d. (o) Same as panel n, but rotated 90 degrees along the medial-lateral axis. (p, r) Images of GAL4 driver line SS77402 in females and males, respectively, shown with the standard neuropil reference, JFRC2018U83. Note the presence of a single TuTuA cell body in each brain hemisphere. (q, s) Images of GAL4 driver line SS77462 in females and males, respectively. Note the presence of a single TuTuA cell body in each brain hemisphere. (t–v) Images of the expression patterns in the brain and VNC of GAL4 driver lines SS77402, SS77462 and SS10166, as indicated. (w) Images of fluorescent in situ hybridization assays to determine the neurotransmitter used by TuTuA_1. Probes used in each panel are indicated. GFP shows the TuTuA_1 cell and Glu and GABA represent probes for vGlut and GAD, respectively (see Methods for details). Scale bar is shown in the left panel.
