Extended Data Fig. 7: Responses of TuTuA subtypes to the activation of female aggression or LC10a cell types.
From: Social state alters vision using three circuit mechanisms in Drosophila
(a–e) Changes in fluorescence intensity as measured by GCaMP6f in the cell body of TuTuA_1 (a, c) or TuTuA_2 (b, d) in response to two 14 s optogenetic stimuli (2 s interval) at 10 Hz (n = 3 – 4). (e) No changes in fluorescence intensity were recorded when stimulating with Chrimson alone (n = 4). Individual trials for a – e are shown in purple (TuTuA_1) or pink (TuTuA_2), mean is in black. (f) Connectivity diagram from Fig. 5a with the connections from pC1d and pC1e. Synapse numbers are indicated on the arrows, which are also scaled according to synapse counts. Arrows indicate putative excitatory connections (cholinergic) and bar endings indicate putative inhibitory connections (SMP054, GABAergic; TuTuA_1 and TuTuA_2, glutamatergic). (g–j) Electrophysiology recordings with the cell types activated with Chrimson are circled in red, and those recorded are in black. Individual trials are in pink (n = 8 trials from 1 cell), mean is in black. (g) Small excitation or negligible response in TuTuA_2 (n = 5 cells) to 15 ms pC1d activation, which was abolished by mecamylamine. (h) Large inhibitory response in TuTuA_2 to 15 ms pC1e activation, which was abolished by mecamylamine (n = 6 cells). (i) Large inhibitory response in TuTuA_2 to 15 ms pC1d/e activation, which was abolished by mecamylamine (n = 8 cells). (j) No evoked response was recorded when stimulating Chrimson in the absence of a LexA driver (n = 6). (k) Latency after stimulus onset (ms). Box-and-whisker plots show median and IQR; whiskers show range. A Kruskal-Wallis and Dunn’s post hoc test was used for statistical analysis. Asterisk indicates significance from 0: ***p < 0.001.
