Extended Data Fig. 10: Controls for inflammation and proliferation.
From: Spatial transcriptomic clocks reveal cell proximity effects in brain ageing
a, Boxplots of the glial activation/inflammation gene signature score for “Far” and “Near” activated/inflamed glia near T cells (activated/inflamed status determined by top 0.2% expression of signature). For activated microglia, n = 15 cells near T cell and n = 16 cells far from T cell. For inflamed oligodendrocytes, n = 64 cells near T cell and n = 64 cells far from T cell. The line indicates the median, the box corresponds to 25th and 75th quartiles, and the whiskers span up to 1.5 times the interquartile range with diamonds showing outlier values. P-values computed using two-sided Mann-Whitney test. b,c, Density plots and histograms showing the nearest distance to different cell types for (b) all activated microglia and (c) all inflamed oligodendrocytes in the coronal section dataset. Numbers of cells for each cell type are listed in parentheses in the legend. d, Schematic showing expected heatmaps (left) for different T cell proximity effect scenarios and the observed heatmap (right) showing the T cell proximity effect on glial cell subtypes (activated/inflamed status, see Methods). e, Schematic showing expected heatmaps (left) for different NSC proximity effect scenarios and the observed heatmap (right) showing NSC proximity effect on terminal cell types in the NSC lineage (astrocytes, oligodendrocytes) and cells not in the lineage (microglia, endothelial cells, pericytes) reproduced from (Fig. 4b).
