Extended Data Fig. 4: Library characterization by long-read sequencing.

a) A histogram of reads of plasmids from PacBio sequencing. The y-axis represents the number of reads of plasmids with a given number of reads (i.e. the bar at 50 on the x-axis is as tall as the number of reads of barcodes with 50 reads). We were able to generate a consensus sequence for any barcode with more than 1 read leaving us with 327,149 possible barcodes. b) A rarefaction plot estimating the overall library complexity, a negative binomial distribution was fit and we estimated a real library complexity of ≈180,000 barcodes. c) A plot of how many mutants (of the possible 19) were in our library at each position (black dashes, left axis) and how many barcodes (green dashes, right axis). d) A heatmap of how many barcodes were characterised for each mutation. e) A histogram of mutants by how many barcodes they had. f) Statistics on the completeness of the library. Overall we had >99% of the mutations in our lookup table.