Extended Data Fig. 8: Related to Fig. 4. Optical readout of sgRNA-containing antigen-specific CD8 T cells in the mouse intestine integrated in the Xenium assay.
From: Tissue-resident memory CD8 T cell diversity is spatiotemporally imprinted
a, Flow cytometry histograms showing quantification of Thy1 and CXCR3 of Cas9eGFP P14 CD8 T cells transduced with Thy1 and Cxcr3-targeting sgRNAs. Representative of two independent biological replicates. b, Dot plot gene expression of the three least expressed genes in the 350 gene panel for all cells in the small intestine, all time points and replicates (n = 8) combined. c, Frequency of CD8 T cells containing one, two or three different sgRNAs. 2 biological duplicates combined. d, Expression of pseudo-gene barcodes in the perturbed day 8 small intestine. e, Capture of pseudo-gene barcodes along the spatial areas of the small intestine defined by IMAP for each perturbation. Two-sample t-test of the mean expression levels, with Benjamini-Hochberg correction applied, *p-value < 0.05. Sample sizes shown in Supplemental Table 12.
