Fig. 3: Characterization of release factor variants using phage assays.
From: Engineering a genomically recoded organism with one stop codon
a, RF1 and RF2 variant strains were challenged with λ phage to assess phage infectivity with genes terminating with TAG, TAA and TGA. PFU, plaque-forming units. b, Release factor variant strains were challenged with µ phage to assess phage infectivity with genes terminating with TAA and TGA only. Data are mean ± 95% confidence interval for n = 3 biological replicates. Unpaired t-test for comparison with E. coli MG1655. NS, not significant; ND, not detected (lower than the measurable detection limit).
