Fig. 4: Engineering tRNATrp to mitigate UGA suppression.
From: Engineering a genomically recoded organism with one stop codon
a, Schematics of wild-type and mutant (A37G) tRNACys(GCA) anticodon loops in S. cerevisiae and wild-type and mutant (A37G) tRNATrp(CCA) anticodon loops in E. coli, depicting loss of anticodon modification and codon recognition in mutant forms. b, Schematic representation of Mj-TyrOTS expression plasmid: IPTG-inducible M. jannaschii tyrosyl-tRNA synthetase (Mj-TyrRS) and accompanying constitutively expressed tRNATyr(UCA). c, Mj-TyrOTS-enabled suppression of UGA within a mCherry–YFP fluorescent reporter expressed in rEc∆2.B2 strains with wild-type or mutant tRNATrp (tW*). Data are mean ± s.e.m. P values for comparison with no Mj-TyrOTS and no Ara (inducer) conditions for each strain by unpaired t-tests (n = 3 biological replicates). d, Stacked mass spectrometry data depicting identities of incorporated residues at UGA from MS-READ analysis of rEc∆2.B2 strains with wild-type or mutant tRNATrp.
