Extended Data Table 1 TGA conversion summary

From: Engineering a genomically recoded organism with one stop codon

  1. Genes and pseudogenes containing TGA codons and those deliberately converted to TAA, deleted, or deactivated (i.e. garK). Release factor 2 gene prfB contains 2 TGAs converted to TAA, counted as one converted gene. Over 300 open reading frames (ORFs) overlapping TGA codons were intentionally mutated as a result of targeted TGA-to-TAA conversions via MAGE. Twenty-one ORFs remain unrecoded, including 3 selenoproteins that contain UGA-specific SECIS elements, 8 transposons, and 10 predicted pseudogenes leftover from extra and unnecessary TGA conversion targeting. See Supplementary Table 1 for full list of TGA conversions. Unrecoded ORFs wither terminal TGAs include: agaA, agaW, pbI, peaD, yrdE, yeeP, ylbI, yjhZ, yibV, yncI, insQ, insI1(2-3), insE1(1-5).
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