Fig. 1: Integrated snRNA-seq reference atlas of the human hypothalamus.

a, Schema of datasets and analyses results. b, UMAP plot of the integrated atlas coloured by principal cell classes. Colouring corresponds to background colours in Fig. 2. c, Details of cluster C2-36 Mid-3 GLU-3. This glutamatergic SIM1-expressing cluster includes AVP neurons of the PVN as well as subclusters from mammillary bodies. Inset, position on the global UMAP that is expanded and coloured by the C4 subcluster. d, Dendrogram of C2-36 Mid-3 GLU-3 with a detailed overview of the subcluster structure. Colouring corresponds to the UMAP in c. The heatmap shows the average expression (in log-normalized scale) of marker genes used for annotation of C3 and C4 clusters. Illustration in a created using BioRender (https://biorender.com); credit: S. O’Rahilly (2024).