Extended Data Fig. 4: Non-neuronal cell types in the human hypothalamus.

a, Spatial mapping of astrocyte populations at the C2 cluster level: cell2location mapping of each of the 3 astrocyte C2 clusters, demonstrating spatially distinct astrocytic populations. b, Spatial mapping of oligodendrocyte populations at the C2 level in the anterior hypothalamus. Cell2location maps the two C2 oligodendrocyte populations to distinct locations in the hypothalamus: C2-8 oligodendrocytes map to the anterior commissure, and C2-7 oligodendrocytes map to the optic chiasm, with both cell types mapping to the lateral preoptic area. c, Mapping of ependymal cell types in the hypothalamus. Cell2location mapping of tanycytes (yellow) and ependymal cell (green) types in the hypothalamus. Tanycytes show distinct mapping to the median eminence and the base of the third ventricle, whereas ependymal cells map to the lining of the third ventricle. d, Log-normalized expression of CRYM and FRZB, both previously identified to be markers of tanycyte subtypes, in the third section below the third ventricle. (Below) Log-normalized expression of DIO2, FZD5, STOML3 and LPAR3, marker genes for tanycytes and/or ependymal cells with (e) complementary smFISH. For each probe the assay was successfully repeated between 1 and 3 times on 3 biological replicates.