Extended Data Fig. 12: Comparison of transgenic expression with gene expression map.
From: Transcriptomic neuron types vary topographically in function and morphology
a, Average expression of atf5b labelled by in situ HCR (cyan) versus knock-in transgenic (Tg) labelling of atf5b+ neurons (magenta). The expression pattern of 3 individual fish was registered onto the mapzebrain.org atlas and averaged. b, Co-labelling of atf5b and Tg(atf5b). A single tectum plane is shown. While not all the atf5b detected with HCR are labelled in the transgenic line, all the transgenic labelled neurons are HCR positive. This results from either variegation of the QF2/QUAS expression, or from neurons expressing the RNA but not the protein product. c, Average expression of cort labelled by in situ HCR (cyan) versus knock-in transgenic labelling of cort+ neurons (magenta). The expression pattern of 3 individual fish was registered onto the mapzebrain.org atlas and averaged. d, Co-labelling of cort and Tg(cort). A single tectum plane is shown. e, Average expression of itpr1b labelled by in situ HCR (cyan) versus knock-in transgenic labelling of itpr1b+ neurons (magenta). The expression pattern of 3 individual fish was registered onto the mapzebrain.org atlas and averaged. f, Co-labelling of itpr1b and Tg(itpr1b). A single tectum plane is shown. g, Average expression of sp5l labelled by in situ HCR (cyan) versus knock-in transgenic labelling of sp5l+ neurons (magenta). The expression pattern of 3 individual fish was registered onto the mapzebrain.org atlas and averaged. A weak expression of sp5l is detected in the caudal hypothalamus in comparison to the Tg line (see k). h, Co-labelling of sp5l and Tg(sp5l). A single tectum plane is shown. i, Co-labelling of sp5l and Tg(sp5l). A single plane of the caudal hypothalamus is shown.
