Fig. 1: Comprehensive characterization of the genetic HCC mouse models.
From: Human-correlated genetic models identify precision therapy for liver cancer
a, Experimental scheme. Conditional genetically engineered mice induced with AAV.TBG.cre virus develop tumours after clonal recombination of genes classically associated with HCC in a TCGA study4. b, Specific combinations of mutations, but not numbers of mutations, drive model-specific features such as survival, tumour proliferation (Ki-67), bleeding from tumour and metastasis in mouse models of HCC. The up arrows represent gain of function (green) and the down arrows represent loss of function (red). T.a.i., time after induction (days); HET, heterozygous; HOM, homozygous. Exact values are provided in Supplementary Table 1. c, Representative images showing that variation in macroscopic and microscopic phenotype depends on combinations of mutations. Glutamine synthetase (GS) was used as an indicator of activated CTNNB1 signalling. Scale bars, 1 cm (macroscopy) and 200 µm (microscopy). Histology for the full range of HCC GEMMs is shown in Extended Data Fig. 3. d, Representative images show lung metastases resembling the primary tumour phenotype as demonstrated by haematoxylin and eosin (H&E) and GS staining. Scale bar, 100 µm. e, Mouse HCC models present common patterns and characteristics used for identification and classification of human HCC based on in-depth histopathological examination. n = 5–7 mice per cohort as indicated by bars.
