Extended Data Fig. 10: HCCOs mimic features of the primary tumour and are suited to investigate drug effects on tumour cells. | Nature

Extended Data Fig. 10: HCCOs mimic features of the primary tumour and are suited to investigate drug effects on tumour cells.

From: Human-correlated genetic models identify precision therapy for liver cancer

Extended Data Fig. 10: HCCOs mimic features of the primary tumour and are suited to investigate drug effects on tumour cells.The alternative text for this image may have been generated using AI.

(a) Schematic of murine HCCO assay pipeline. HTP = high-throughput, GEMM = genetically-engineered mouse model, HCC = hepatocellular carcinoma, HCCOs = HCC organoids. (b) HCCOs keep characteristics of primary tumour tissue such as accumulation of beta-catenin (CTNNB1), proliferation marker Ki67, differentiation marker HNF4a, glutamine synthetase (GS) and MYC expression; representative images of single organoids from a bulk culture from a single organoid line and of single tumours from multiple autochthonous tumours within a single mouse. Scale bars equal 100 µm. NT = non-tumour, T = tumour. (c) The transcriptional phenotype of HCCOs differed from the original tumours of the same Cohort, likely due to the simplified nature of HCCOs as an epithelial-cell-only model as well adaptive response to the culture conditions. Note that all GEMMs and HCCOs are mutated/overexpressing of both CTNNB1/Myc respectively.

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