Extended Data Fig. 4: Mutational processes in murine stem cells.
From: Clonal dynamics and somatic evolution of haematopoiesis in mouse
a) Signature extraction overview. Trinucleotide spectra from all single-base substitutions (SBS) (top), were used for signature extraction as described in the Methods. Three signatures identified as SBS1, SBS5, and SBS18 best described the catalogue of mutations observed (cosine similarity=0.997). b) Linear mixed-effect regression of signature-specific mutation burdens observed in colonies. Shaded areas indicate the 95% confidence interval. c) Signature attribution in phylogenies. Individual branches of HSC phylogenies are overlaid with signature contribution proportions. SBSs assigned to each branch were fit to SBS1, SBS5 or SBS18. d) Signature-specific mutation accumulation in all branches across phylogenies. Early-life branchpoints, located at the top of a given phylogeny, and shown as an inset.
