Extended Data Fig. 13: Platelet-mediated suppression of T cells and anti-metastatic immunity.
From: Aspirin prevents metastasis by limiting platelet TXA2 suppression of T cell immunity
a, Schema showing isolation of platelets and transwell co-culture with proliferation dye CellTrace Violet (CTV)-labelled naïve CD8+ T cells. Transwell plates were pre-coated with anti-CD3 and soluble anti-CD28 antibodies to stimulate CD8+ T cells. b, Flow cytometry plots showing proliferation and activation of naïve and Arhgef1-decifient T cells in (a) as determined by dilution of CTV dye and expression of CD44. c, Abundance of TXB2 as determined by enzyme-linked immunosorbent assay (ELISA) of transwell co-culture supernatants of cells in (b) at day 5 post-stimulation. n = 4 independent replicates per condition. d, Effect of platelet depletion on the frequency of metastases on lungs of Cd4Cre (cWT, vehicle = 18 and R300 = 19) and Arhgef1fl/fl Cd4Cre (cKO, vehicle = 18 and R300 = 20) mice 17 days after i.v. B16 injection. e, Frequencies of platelets in blood as determined by flow cytometry from animals in (d). cWT treated with vehicle (n = 5) and R300 (n = 6), and cKO treated with R300 (n = 8). Data are representative of two (b, c, e) or pooled from two (d) independent experiments. One-way ANOVA with Tukey multiple comparisons test. Graphs show mean ± s.e.m.
