Extended Data Fig. 1: Reduced cancer metastasis in animals lacking ARHGEF1.
From: Aspirin prevents metastasis by limiting platelet TXA2 suppression of T cell immunity
a, Representative flow cytometry plots (left) and number (right) of mCherry+ CD45– tumour cells in lungs of WT and Arhgef1 KO littermates 17 days after intravenous (i.v.) injection of B78ChOva-mCherry cells. b, Representative photomicrographs of haematoxylin and eosin (H&E) stains of lung sections from wildtype and Arhgef1-deficient animals 17 days after intravenous injection of syngeneic LL/2 lung adenocarcinoma cells. Arrows indicate metastatic deposits. c, Representative photomicrographs of H&E stains of liver sections from wildtype and Arhgef1-deficient animals 11 days after intrasplenic injection of syngeneic B16-F10 melanoma cells. Arrows indicate metastatic deposits. d, Cumulative area of primary mammary tumours arising in Arhgef1-WT (n = 15) and -KO (n = 9) MMTV-PyMT female littermate mice at the indicated ages (left) and survival time to cumulative area of 2.25 cm2, at which time point lungs were harvested for histopathology analysis. Log-rank test; P = 0.31. e, Area of tumours of WT (n = 7) and Arhgef1 KO (n = 5) littermates was measured at the indicated time points following subcutaneous implantation of MC38 colorectal adenocarcinoma cells. Data are representative of five (a) and two (e) independent experiments or pooled from three (d) independent experiments. Unpaired two-tailed Student’s t test (a). Graphs show mean ± s.e.m.
