Extended Data Fig. 3: Immune responses in tumour-bearing lungs of animals bearing a conditional deletion of ARHGEF1 in T cells.
From: Aspirin prevents metastasis by limiting platelet TXA2 suppression of T cell immunity
a, Flow cytometry plots (left) and replicate measurements (right) of the number of mCherry+ CD45– tumour cells in lungs of Cd4Cre (cWT = 9) and Arhgef1fl/fl Cd4Cre (cKO =10) animals 17 days after intravenous (i.v.) injection of B78ChOva cells. b, Quantification of CD4+ (left) and CD8+ (right) T cells from tumour-bearing lungs of cWT (n = 21) and cKO (n = 20) mice 17 days after i.v. injection of tumour cells. c, Relative frequency of CD8+ T cells within all 15 phenograph clusters (data for clusters 4, 9, 10 and 14 also shown in Fig. 2g, as noted in Source Data) from lungs of B78ChOva tumour-bearing and non-tumour-bearing mice of the indicated genotypes (tumour-bearing cWT, n = 11; tumour-bearing Arhgef1 cKO, n = 10; cWT, n = 3; cKO, n = 4). d, PD-1 and TIGIT expression on effector CD8+ T cells within tumour-bearing lungs of animals in (c) of the indicated genotypes. Representative flow cytometry plots (left) and replicate measurements (right). Data are representative of five (a) and two (c, d) independent experiments or pooled from three (b) independent experiments. Unpaired two-tailed Student’s t test (b); 2-way ANOVA with Tukey multiple comparisons test (c); One-way ANOVA with Tukey multiple comparisons test (d) and two-tailed Mann-Whitney U test (a). Graphs show mean ± s.e.m.
