Fig. 3: Effect of manipulation of VGAT- and VGluT2-expressing MRN neurons on valence.
From: A subcortical switchboard for perseverative, exploratory and disengaged states
a, Schematic of the experimental design for a self-stimulation test. b, Preference for returning to the opto-linked nose-poke port (100 × (number of entries into the opto-linked nose-poke port − number of entries into the non-stimulation nose-poke port)/total number of entries into both nose-poke ports) in tdTomato control mice (n = 9) and mice with activation or suppression of VGAT+ (n = 5 and 6 mice) and VGluT2+ (n = 5 and 9 mice) MRN neurons. c, Schematic of the TMT aversion test. d, Number of approaches of the aversive TMT-coated object in control mice compared to mice with activation or suppression of VGAT+ and VGluT2+ MRN neurons. e, Escape probability after approaching a TMT-coated object in control mice (n = 9) compared with mice with VGluT2+ suppression (n = 13 mice), VGluT2+ activation (n = 11), VGAT+ suppression (n = 11) and VGAT+ activation (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001. In b,d,e, bars indicate median, error bars are bootstrapped standard error and circles represent individual experimental sessions. See Extended Data Table 1 for statistics.
