Fig. 2: MINFLUX tracking of the bidirectional transport of Imp α.
From: Overlapping nuclear import and export paths unveiled by two-colour MINFLUX
a, Schematic of the concurrent import and export of Imp α labelled with JF549. The NPC structure was adapted from refs. 44,45, AAAS. b, Target coordinate pattern. The MINFLUX 3D donut was scanned in a seven-point octahedral pattern (black dots) for the Imp α–JF549 tracking algorithm, yielding successive localizations (gold stars). The NPC structure was adapted from ref. 28, Springer Nature, ref. 46, AAAS and the RCSB Protein Data Bank47. c,d, Unfiltered two-colour MINFLUX localization data obtained in the presence of transport mix. NPC localizations (blue; NbGFP–HMSiR; see Fig. 1) were collected for 20 min, and these were followed by tracking localizations (coloured z scale, Imp α–JF549) collected for 20 min. Views from the cytoplasm (c; xy) and the side (d; xz) for two different cells are shown. e, Tracks (magenta) that satisfied MINFLUX filtering criteria (see Methods and Extended Data Fig. 6) overlaid onto four NPC scaffolds (blue).
