Extended Data Fig. 4: Determining the z-scaling factor for MINFLUX measurements.
From: Overlapping nuclear import and export paths unveiled by two-colour MINFLUX
a, Averaged structure for NPCs from uncorrected MINFLUX measurements (37 cells, 541 NPCs, 82,331 HMSiR localizations). b,c, The z (b) and radial (c) distributions for the data in a. The distance between the two peaks in b yields the ring separation (76.8 ± 0.8 nm), and the peak in c is considered the radius of the pore (51.1 ± 1.4 nm). The data/results for a-c are summarized in Supplementary Table 2 (row 8). d, Averaged structure for NPCs from astigmatism measurements of the pore scaffolds used for the alignment of mEosEM data in Fig. 4 and Extended Data Fig. 9 (129 cells, 1453 NPCs, 17,234 HMSiR localizations). e,f, The z (e) and radial (f) distributions for the data in d. The data/results for d-f are summarized in Supplementary Table 2 (rows 2–5). g, Averaged structure for NPCs from corrected MINFLUX measurements. The data in a were corrected by multiplying all z values by 0.67. This factor was determined as indicated in Supplementary Table 2 (note ‘e’). The images on the left are shown in Fig. 1g,h. h,i, The z (h) and radial (i) distributions for the data in g. Note that the radial distribution for the corrected MINFLUX data (i) is identical to c. The data/results for g-i are summarized in Supplementary Table 2 (row 9).
