Extended Data Fig. 2: Human neutrophils and neutropenia mouse models.
From: Matrix-producing neutrophils populate and shield the skin
a, Cytometry plots showing the frequency of COL3+ neutrophils of human skin and blood. Right, quantification of percentages. Bars show mean ± s.e.m. from 5 blood and 6 skin samples. P-value determined by two-sided unpaired Student’s t-test. b, Image of a cleared human skin sample stained for neutrophils and Col3a1. White arrowheads show COL3-negative neutrophils, and yellow arrowheads COL3+ neutrophils. The image is representative of 3 donors. c, Representative images of Col3a1 staining in sorted neutrophils from human blood, spleen, lungs, and skin. Negative control IgG staining is shown for human skin neutrophils. Right, per cent Col3a1+ neutrophils in barrier and non-barrier human tissues. Data are from 3 human specimens per tissue. P-value determined by one-way ANOVA with multigroup comparison test. d, Representative cytometry plots and gating strategy for neutrophils across the indicated tissues (iDTR model). e, Neutrophil numbers in tissues by flow cytometry of the three neutropenia mouse models and their respective controls (IgG, MRP8WT; iDTR and LyzMWT; Mcl1flox, respectively). Mice were treated intraperitoneally with either anti-Ly6G (1A8, 3 µg/g) or control IgG (3 µg/g) antibodies or diphtheria toxin (DT, 10 μg/kg) for two consecutive days and tissues were collected on the third day. The number of mice (n) is displayed in the figure. P-values were determined by a two-sided unpaired Student’s t-test.
