Extended Data Fig. 7: Single-particle cryo-EM data processing for FabE and FabB complexes.
From: A coronavirus assembly inhibitor that targets the viral membrane protein
a, Representative motion-corrected electron micrograph of FabE complex embedded in vitreous ice. Scale bar = 20 nm. b, Representative reference-free 2D class averages. c, Gold-standard Fourier shell correlation (FSC) curve generated from the independent half maps contributing to the 3.3 Å global resolution density map of the FabE complex. d, Local resolution filtered EM density map for the refined FabE complex (left), and central-slice of the complex (right), coloured according to local resolution which was calculated in CryoSPARC. e, Representative motion-corrected electron micrograph of FabB complex embedded in vitreous ice. Scale bar = 20 nm. f, Representative reference-free 2D class averages. g, Gold-standard Fourier shell correlation (FSC) curve generated from the independent half maps contributing to the 3.2 Å global resolution density map of the FabB complex. h, Local resolution filtered EM density map for the refined FabB complex (left), and central-slice of the complex (right), coloured according to local resolution which was calculated in CryoSPARC. Location of the CIM-834 binding pocket is circled.
