Fig. 4: EM dataset.
From: Functional connectomics spanning multiple areas of mouse visual cortex
a, Top view of EM dataset (grey) registered with the in vivo 2P structural dataset (vasculature in red and GCaMP in green). Area borders calculated from calcium imaging are shown as black lines. The two portions of the dataset are separated by a dashed line. Scale bar, 500 μm. Mouse drawing adapted from from Mahalingam et al.64, CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/). b,c, Top view of small region showing the quality of the fine alignment and its robustness to large folds shown in c (the dataset is available at https://ngl.microns-explorer.org/#!gs://microns-static-links/mm3/data_fig/4b.json). Scale bars, 5 μm. d, Montage of a single section showing the coverage from pia to white matter and across three different cortical regions. Scale bar, 100 μm. e, Example of a single tile from the section shown in in d, with dashed squares representing the locations in f–h. Scale bar, 5 μm. f,g, Examples of excitatory synapses indicated with arrowheads (dataset available at https://ngl.microns-explorer.org/#!gs://microns-static-links/mm3/data_fig/4f.json (f) and https://ngl.microns-explorer.org/#!gs://microns-static-links/mm3/data_fig/4g.json (g)). h, Example of an inhibitory synapse (arrowhead) (dataset available at https://ngl.microns-explorer.org/#!gs://microns-static-links/mm3/data_fig/4h.json).
