Fig. 6: Integrated analysis resources and examples.
From: Functional connectomics spanning multiple areas of mouse visual cortex
a–e, Cell body locations and cell-are type classifications, all nucleus detections shown in light grey. a, Non-neuronal cells, manually typed (dark outlines) and classifier-based (no outline)6. OPC, oligodendrocyte precursor cell. b, Excitatory cells, labelled by unsupervised clustering of morphological features4 (dark outline) and a model based on those labels6. L2, layer 2; L3, layer 3; L4, layer 4; L5ET, layer 5 extratelencephalic; L5IT, layer 5 intratelencephalic; L5NP, layer 5 near-projecting; L6CT, layer 6 cortico-thalamic; L6IT, layer 6 intratelencephalic; L6WM, layer 6 white matter. c, Inhibitory cells, classified by human experts4 and trained models6. d, Neurons registered to in vivo functional traces. e, Proofreading status of neurons in subvolume 65: black dots (fully proofread), red (cleaned of false merges but potentially incomplete) and blue (dendrites cleaned/extended). f, The number of output synapses per neuron shown in e versus the fraction mapped to a single postsynaptic soma, coloured by cell class. g, A fully proofread pyramidal cell (nucleus ID: 294657, segment ID: 864691135701676411) with postsynaptic soma locations shown as coloured dots (by cell class). Cells with functionally co-registered regions are outlined in dark green. h, Quantification of synapses associated with different categories of postsynaptic cells. The first column shows the fraction that map to a single postsynaptic soma. The second column shows the fraction of those that are excitatory or inhibitory. The third column shows the fraction of cells that are in each sub-class based on the model shown in b,c. The fourth column shows the proportion that map to functionally co-registered cells. The cell and its synapses are viewable at https://neuroglancer-demo.appspot.com/#!gs://microns-static-links/mm3/data_fig/6f.json. i, EM image (i) and corresponding image from the 2p structural stack (j) centred on the cell shown in g (yellow circle). Red arrowheads indicate blood vessels. k, Functional responses of the presynaptic (presyn) neuron (g; yellow) and its functionally co-registered postsynaptic (postsyn) targets. Heat maps show average ΔF/F traces for the presynaptic neuron and postsynaptic targets, sorted by synaptic strength, in response to oracle clips from functional scans.
