Extended Data Fig. 1: Region-specific effect of chronic stress or antidepressants on brain autophagy.
From: Stress dynamically modulates neuronal autophagy to gate depression onset
a, b, Antidepressant dose regimen of paroxetine (a1, LHb, nVeh/Pxt = 5/7; nVeh/Pxt = 7 for the rest of brain regions; a2, nVeh/Pxt = 5/group) and ketamine (b1, nVeh/Pxt = 5/6, for LS, nVeh/Pxt = 5 for the rest of brain regions; b2, nVeh/Pxt = 5 for MRN, nVeh/Pxt = 5/6 for the rest of brain regions) treatment (i.p.) enhances autophagy (i.e., decreased p62 and increased Beclin-1) in LHb, but does not affect autophagy in vHippo, mPFC, VTA, LH, NAc, LS, DRN and MRN of CRS mice. Two-sided Mann-Whitney test (a1, a2, b1 and b2). Data are mean ± s.e.m. LHb, lateral habenula; vHippo, ventral hippocampus; VTA, ventral tegmental area; NAc, nucleus accumbens; mPFC, medial prefrontal cortex; LH, lateral hypothalamus; LS, lateral septum; DRN, dorsal raphe; MRN, median raphe; Veh, vehicle; Pxt, paroxetine; Ket, ketamine; CRS, chronic restraint stress. Schematics in a,b adapted from ref. 31, Elsevier.
