Extended Data Fig. 7: An eSNP in the promoter of the GCGR gene was identified after selection of chickens (outbred) for high or low blood glucose. Related to Fig. 3.
From: Constitutively active glucagon receptor drives high blood glucose in birds
a, Serum GCG and insulin levels detected in 60-day-old male C/C and its littermates C/G and G/G of F3 generation. n = 4/group. b,c, Lipolysis genes (Hsl, Lpl and Atgl) (b), lipogenesis genes (Acc, Acot7, Fasn, Pparγ and Cebpα) expression (mRNA) (c) detected in 60-day-old male C/C and littermates C/G and G/G of F3 generation. n = 4/group. d,e, Serum (d) and hepatic NEFAs, and TGs (e), were detected in 60-day-old male C/C and littermates C/G and G/G of F3 generation. n = 4/group. f-j, GCGR gene (f), lipolysis genes (Hsl, Atgl, Lpl) (g), lipogenesis genes (Acc, Acot7, Fasn) (h) and thermogenesis genes (Pparγ, Pgc1α) (i), fatty acid oxidation genes (Cpt1a, Cpt1b) (j) expression levels in subcutaneous adipose tissue (SAT) of 60-day-old male C/C and its littermates C/G and G/G. Data are mean ± SEM from at least three independent experiments, performed in technical triplicate, and analysed by one-way ANOVA and Tukey’s post hoc test (a-j).
