Extended Data Fig. 9: CGRPCEA projection stimulation reactivates flavour representations in the amygdala, and CGRP neuron ablation impairs delayed CFA learning.
From: A neural mechanism for learning from delayed postingestive feedback
Panels a–e show that CGRPCEA projection stimulation reactivates flavour representations in the amygdala. a, Reconstruction of recording trajectories registered to the Allen CCF for mice with CGRPCEA projection stimulation (n = 32 shanks from 8 mice). b, Average spiking of individual neurons during the CGRPCEA projection stimulation conditioning experiment (n = 1,221 neurons from 8 mice). c, Average spiking of the novel flavour-preferring (n = 354 neurons), water-preferring (n = 129 neurons), and nonselective (n = 738 neurons) populations across the entire experiment. d, Average spiking of the novel flavour-preferring, water-preferring, and nonselective populations during individual bouts of CGRPCEA projection stimulation (same sample sizes as c). e, Neural trajectories in PC-space for novel flavour consumption, water consumption, and CGRPCEA projection stimulation. Panels f,g show that genetic ablation of CGRP neurons by taCasp3-TEVp impairs delayed CFA learning. f, Example CGRP immunoreactivity data confirming genetic ablation of CGRP neurons by taCasp3-TEVp. This is an expanded version of Fig. 3p. g, CGRP neuron ablation mice show significantly higher acceptance of the conditioned flavour following LiCl-induced CFA when compared to wild type controls (n = 6 taCasp3 mice, 7 control mice). Panels h–j show that CGRP neuron stimulation-activated CEA neurons are also activated by LiCl injection. h, Schematic. i, Average spiking during CGRP neuron stimulation and then during LiCl-induced malaise (n = 821 neurons from 4 mice). j, Average spiking of the CGRP neuron stimulation-activated neurons (n = 189 neurons) and other neurons (n = 632 neurons) during LiCl-induced malaise. Shaded areas represent mean ± s.e.m. Inset box plots show the 10th, 25th, 50th, 75th, and 90th percentiles. *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001. See Supplementary Table 2 for details of statistical tests and for exact P values.
