Extended Data Fig. 10: Postingestive CGRP neuron activity is necessary and sufficient to stabilize flavour representations in the amygdala upon memory retrieval.
From: A neural mechanism for learning from delayed postingestive feedback
Panels a,b relate to Fig. 4b–d. a, Proportion of flavour-preferring neurons classified separately on conditioning or retrieval day (n = 8 mice). b, Population trajectories for flavour consumption, water consumption, and CGRP neuron stimulation. Panels c–e relate to Fig. 4e. c, Average spiking of all individual neurons for the CGRPCEA projection stimulation experiment (n = 1,042 neurons from 8 mice). d, Analogous to a, but for mice with CGRPCEA projection stimulation (n = 8 mice). e, Average spiking of the novel flavour-preferring neurons with the highest 10% CGRPCEA response magnitudes and of the remaining novel flavour-preferring neurons. Panels f,g show that LiCl-induced malaise stabilizes the flavour representation upon retrieval, and that this is impaired by CGRP neuron ablation. f, For control mice, average spiking of the novel flavour-preferring population (n = 279 neurons from 4 mice). g, Analogous to f, but for mice with CGRP neuron ablation (n = 109 neurons from 4 mice). Panels h–k relate to Fig. 4f. h, Average spiking of all individual neurons (n = 924 neurons from 7 mice). i, Proportion of flavour-preferring neurons classified separately on novel or familiar day (n = 7 mice). j, Average spiking of the initially water-preferring population (n = 160 neurons from 7 mice; classified on novel day) during flavour consumption. k, Population trajectories for flavour and water consumption. l, Time-courses along the PC2 axis during consumption following CGRP neuron stimulation conditioning (from b) and familiarization (from k). Error bars and shaded areas represent mean ± s.e.m. Inset box plots show the 10th, 25th, 50th, 75th, and 90th percentiles. NS, not significant, *P ≤ 0.05, **P ≤ 0.01. See Supplementary Table 2 for details of statistical tests and for exact P values.
