Fig. 3: Postingestive CGRP neuron activity preferentially reactivates the representation of a recently consumed flavour in the amygdala.
From: A neural mechanism for learning from delayed postingestive feedback
a, Hypotheses for how the amygdala associates temporally separated flavour and malaise signals. b, Schematic of the CGRP neuron-stimulation recording strategy. c, Reconstruction of recording trajectories registered to the Allen CCF. Each line represents one shank of a four-shank Neuropixels 2.0 probe (32 shanks from 8 mice). d, Average spiking of all individual neurons (n = 1,104 single units and multiunits from 8 mice). e, Average spiking of novel-flavour-preferring (n = 373), water-preferring (n = 121) and nonselective (n = 610) populations. f, Left, average spiking of individual neurons during CGRP neuron-stimulation bouts. Right, population averages (same sample sizes as e). g, Example of a multinomial logistic regression decoder session. h, Average decoder posterior time locked to CGRP neuron stimulation (mean across 6 mice). i, Average reactivation rates of novel flavour or water representations (n = 6 mice). j, PCA schematic. k, Population trajectories for novel-flavour consumption, water consumption and CGRP neuron stimulation. l, Trajectories for individual example mice. m, Schematic of the LiCl-induced-malaise recording strategy. n, Average spiking of novel-flavour-preferring (n = 280 neurons from 4 mice), water-preferring (n = 80) and nonselective (n = 218) populations. o, Average reactivation rates of novel flavour or water representations (n = 4 mice). p, Example CGRP immunoreactivity data confirming the ablation of CGRP neurons in the PB (outlined in green). Scale bar, 100 µm. q, Analogous to n, but for mice in which CGRP neurons were ablated (n = 124 novel-flavour-preferring, 20 water-preferring, 256 nonselective neurons from 4 mice; all statistical tests NS). r, Analogous to o, but for mice in which CGRP neurons were ablated (n = 4 mice). Shaded areas represent the mean ± s.e.m. Inset box plots show the 10th, 25th, 50th, 75th and 90th percentiles. **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. See Supplementary Table 2 for details of statistical tests and for exact P values.
