Extended Data Fig. 2: Activation of the LS during novel flavour consumption blocks malaise-driven amygdala activation and interferes with CFA acquisition.

a, Schematic and example hM3D-mCherry expression data for the bilateral chemogenetic LS activation experiment. CNO was delivered 45-min before the experiment began to ensure that the LS was activated throughout consumption. b, Retrieval test flavour preference for the experiment described in a (n = 18 hM3D mice, 12 YFP mice). c, Schematic of the LS activation FOS time point (n = 12 mice per group for d–g). As in a, CNO was delivered 45-min before the experiment began, and the flavour was novel for both groups. d, Comparison of LS FOS (including the entire ‘Lateral septal complex’ in the Allen CCF) for individual YFP and hM3D mice, confirming strong activation by hM3D. e, Comparison of CEA FOS for individual YFP and hM3D mice, showing reduced malaise-driven activation in hM3D mice. f, Correlation between the average FOS⁺ cell count of each brain region for hM3D versus YFP mice. The amygdala network (from Fig. 1f, g; n = 12 regions), septal complex (n = 4 regions), and all other regions (n = 114 regions) are shown separately. g, Visualization of the difference in FOS⁺ cell density across YFP versus hM3D mice with Allen CCF boundaries overlaid. Error bars represent mean ± s.e.m. Shaded areas represent linear fit estimate ± 95% confidence interval. **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. See Supplementary Table 2 for details of statistical tests and for exact P values. See Supplementary Table 1 for list of brain region abbreviations.

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