Extended Data Fig. 4: Comparison of in silico and in vivo receptive field centers.
From: Functional connectomics reveals general wiring rule in mouse visual cortex
a, Visual comparison of Spike-Triggered Average receptive fields (STAs) generated from in vivo responses to a sparse noise stimulus (top row) vs STAs generated from in silico responses to the same stimulus (bottom row) for three animals (blue, orange, and green). The black cross represents the model readout location. Examples are randomly chosen from the top 44% of neurons remaining after a threshold on in vivo STA quality is applied. b, Model readout location vs in vivo STA center for azimuth coordinate (left) and elevation coordinate (right). c, in silico STA center vs in vivo STA center for azimuth coordinate (left) and elevation coordinate (right). d-i, Retinotopic maps for animal id: 29755. d, Retinotopic maps generated from in vivo STA centers with top 44% of neurons after an in vivo STA quality threshold is applied. Left: Azimuth retinotopic map, each dot represents one neuron in the cortical space, the color represents the azimuthal visual angle of its receptive field center. Middle: Elevation retinotopic map. Right: The coherence of the retinotopic map is visualized as a scatterplot of the pairwise cortical distance vs. the pairwise retinotopic distance for 10,000 randomly selected neuron pairs within the dotted circular region in the retinotopic maps. The coherence is quantified as the Spearman’s rank correlation coefficient between the distances. e, Retinotopic maps generated from in vivo STA centers with the bottom 25% neurons based on the quality of the in vivo STA. f, g, Retinotopic maps generated from in silico STA centers for the same neurons in d and e. h, i, Retinotopic maps generated from the digital twin model readout location for the same neurons in d and e. Colorbar: degree of visual angle for both azimuth and elevation coordinates. Anatomical axes: A = anterior, P = posterior, M = medial, L = lateral. Scale bar: 100 μm. j, k, Analysis in Fig. 3a,b repeated with in silico STA centers instead of model readout location.
