Extended Data Fig. 5: Example of connected, ADP, and same area controls for the area/layer analysis in Fig. 4.

For all panels, a single presynaptic neuron skeleton is shown as in Fig. 2c with its postsyns (black dots), ADP controls (red dots), same area controls (blue dots), and all other functionally matched neurons (gray dots). The location of the presynaptic soma is indicated by the orange triangle. The vertical gray dotted line is the V1 and HVA boundary, with V1 on the left, and HVA on the right. The scale bars are 100 μm a, The V1 L2/3 neuron reproduced from Fig. 2c with its V1 → V1 postsyns and controls (left), and shown again with only its V1 L2/3 (left middle), V1 L4 (right middle) and V1 L5 (right) postsyns and controls. b, The V1 L2/3 neuron reproduced from Fig. 2c with its V1 → HVA postsyns and controls (left), and shown again with only its HVA L2/3 (left middle), HVA L4 (right middle) and HVA L5 (right) postsyns and controls. c, The HVA L5 neuron reproduced from Fig. 2c with its HVA → HVA postsyns and controls (left), and shown again with only its HVA L2/3 (left middle), HVA L4 (right middle) and HVA L5 (right) postsyns and controls. d, The HVA L5 neuron reproduced from Fig. 2c with its HVA → V1 postsyns and controls (left), and shown again with only its V1 L2/3 (left middle), V1 L4 (right middle) and V1 L5 (right) postsyns and controls.