Fig. 2: The poly(A) tail of mRNA-1273 is extended in macrophages in vivo and in vitro.
From: Re-adenylation by TENT5A enhances efficacy of SARS-CoV-2 mRNA vaccines
a, mRNA-1273 poly(A) length distribution in tissues surrounding vaccine injection sites up to 24 h after intramuscular administration. dLNs, draining lymph nodes. b,c, mRNA-1273 poly(A) length distribution in mBMDMs (b) or hMDMs (c) incubated with mRNA-1273 LNPs for up to 96 h. d, BNT162b2 poly(A) length distribution in mBMDMs incubated with BNT162b2 LNPs for up to 72 h. e, In vitro translation assay (3 h) using reticulocyte lysate for mRNA-1273 and BNT162b2. Three micrograms of mRNA was used. Protein was measured by ELISA. Mean ± s.e.m. indicated in red. P values calculated with two-sided t-test. n = 4 independent biological experiments. For a–d, eDRS-measured poly(A) tail lengths with median values and fractions of shortened or elongated tails are shown. For a–c, reads containing (blue) or lacking (green) an mΨCmΨAG sequence are shown separately. Bottom panels show the relative abundance of each group, with group sizes indicated. P values calculated with two-sided Wilcoxon test (on reads lacking mΨCmΨAG) with Benjamini–Hochberg correction. Data represent two (a,c,d) or three (b) independent experiments. See also Extended Data Figs. 2 and 3.
