Extended Data Fig. 8: Analysis of multi-localized proteins and assemblies.
From: Multimodal cell maps as a foundation for structural and functional genomics
a) Number of distinct assemblies per protein, defined as the number of distinct paths to the root of the U2OS cell map hierarchy (shown in Fig. 2b). b) Tripartite localization of XAB2 in the cell map visualized in circle-packing mode. XAB2 is highlighted by red circle and the cell map assemblies in which it participates are highlighted with orange borders. Cytosol and nucleus are filled as blue and pink respectively. c) Top: Immunofluorescence images of XAB2 (middle) and representative interacting partners in cytosol (WDR83, left) or nucleus (DHX8, right). These proteins are immunostained (green) with cytoskeleton counterstain (red). Scale bar, 2.5 µm. Bottom: Biophysical interaction partners of XAB2 with cell map localizations in cytosol and membrane (turquoise box) or nucleus (pink box). d) Cell map coloured to indicate multi-localized assemblies (red nodes). The multiple containing assemblies are indicated in each case (red edges). Dashed box denotes the assembly detailed in text and in panel f–g. e) Number of assemblies with single (left) versus multiple (right) localizations. f) Biophysical interaction data for Amyloid Precursor Protein (APP) complex. g) Immunofluorescence images for proteins in APP complex, immunostained (green) with cytoskeleton counterstain (red). Scale bar, 2.5 µm.
