Fig. 3: Global analysis of assemblies with SEC–MS.
From: Multimodal cell maps as a foundation for structural and functional genomics
a, Overview of the SEC–MS experiment. b, The elution fractions (columns) for proteins (rows) in representative cell map assemblies. The intensity (colour) represents the relative protein abundance in each fraction, scaled in the range [0–1] for each protein across all fractions. Previously undocumented assemblies are indicated in purple font. c, The distribution of SEC–MS Pearson correlations among pairs of proteins in assemblies, shown separately for small assemblies, medium assemblies and random pairs. Significant differences from random pairs are indicated, determined using one-sided Wilcoxon rank-sum tests; ****P < 0.0001. d, Cell map with assemblies coloured on the basis of the significance of validation by SEC. The assembly colour indicates the FDR as determined using one-sided Wilcoxon rank-sum tests comparing SEC co-elution profiles for protein pairs in that assembly versus protein pairs that do not co-occur in any assembly under the root.
